Simple, Inexpensive RNA Isolation and One‐Step RT‐qPCR Methods for SARS‐CoV‐2 Detection and General Use – Wiley

INTRODUCTION
Detection of viral RNA in patient samples provides a sensitive means of diagnostic testing for SARSCoV2 infection. The most common method for RNA detection involves reverse transcription (RT) of sensestrand viral RNA followed by the quantitative polymerase chain reaction (RTqPCR), also referred to as realtime RTPCR (rRTPCR) (Centers for Disease Control and Prevention, 2020). The essentials of RTqPCR have been extensively reviewed elsewhere (see Current Protocols article: Fraga, Meulia,…

Click here to view the original article.