Cas9 modification can improve efficiency of gene editing – The Science Board

Gene editing nucleases such as CRISPR/Cas9 can efficiently create double-stranded breaks at a target locus in the genome. Often, the double-stranded breaks are subsequently repaired by the nonhomologous end-joining mechanisms, leading to insertions and deletions (indels). One strategy, called homology directed repair (HDR), is used to achieve gene knock-in models (targeted gene insertion).
These gene editing techniques are relatively efficient in single-strand annealing, as is common with Streptococcus…

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