Science
Positive dielectrophoresis–based Raman-activated droplet sorting for culture-free and label-free screening of enzyme function in vivo – Science Advances
The potential of Raman-activated cell sorting (RACS) is inherently limited by conflicting demands for signal quality and sorting throughput. Here, we present positive dielectrophoresis–based Raman-activated droplet sorting (pDEP-RADS), where a periodical pDEP…
INTRODUCTION
A single cell is the basic unit of function for life on Earth. A single-cell Raman spectrum (SCRS) consists of thousands of resonance or nonresonance Raman peaks, which individually or in combination can model a particular biochemical or metabolic phenotype of the cell; thus, SCRS can capture the metabolic state of a cell just like a function-based instant photo (1). By sorting cells via SCRS, Raman-activated cell sorting (RACS) holds great promise as a powerful platform for screen…
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